KISHENKO V., LEVCHUK I., GOLUBETS O. DETERMINATION OF BENZO[A]PYRENE IN SUNFLOWER SEEDS, EDIBLE OILS AND DERIVED PRODUCTS MANUFACTURED IN UKRAINE BY LIQUID CHROMATOGRAPHY USING FLUORESCENCE DETECTOR AND GAS CHROMATOGRAPHY USING MASS SELECTIVE DETECTOR

2011 – Zurnal Hromatografichnogo Tovarystva  – V. ХІ. – № 1-4. –  P. 5-11

UDC 664: 665.1/7:539:542/543

KISHENKO V., LEVCHUK I., GOLUBETS O.

DETERMINATION OF BENZO[A]PYRENE IN SUNFLOWER SEEDS, EDIBLE OILS AND DERIVED PRODUCTS MANUFACTURED IN UKRAINE BY LIQUID CHROMATOGRAPHY USING FLUORESCENCE DETECTOR AND GAS CHROMATOGRAPHY USING MASS SELECTIVE DETECTOR - article

The method for qualitative and quantitative determination of benzo[a]pyrene in edible oils and oilseeds by HPLC using fluorescence detector has been developed. The sample preparation comprises alkaline hydrolysis of the sample, liquid-liquid extractionof PAH and Florisil clean-up procedure. The benzo[b]chrysene was used as internal standard for quantification. Confirmation of positive results has been carried out by GC-MS. The method described has been used for benzo[a]pyrene content determination in edible oils, oilseeds and derived products manufactured in Ukraine. The benzo[a]pyrene content in 24 samples of sunflower seeds and in 41 samples of crude sunflower oil from different regions of Ukraine ranged from 0.5 to 10.2 μg/kg of fat and from 0.8 to 21.0 μg/kg respectively.

Keywords: polycyclic aromatic hydrocarbons, benzo[a]pyrene, high performance liquid chromatography, gas chromatography-mass spectrometry, edible oils, oilseeds.