Shandrenko S. G., Golovin A. S., Dmytrenko M. P., Yurchenko A. I., Babycheva O. F. The computer registration and analysis of thin-layer chromatography results

2002 – Zurnal Hromatografichnogo Tovarystva – V. II – № 4. – P. 22-30

UDC 681.3.06:541.183

SHANDRENKO S. G., GOLOVIN A. S., DMYTRENKO M. P., YURCHENKO A. I.,      BABYCHEVA O. F. 

THE COMPUTER REGISTRATION AND ANALYSIS OF  THIN-LAYER CHROMATOGRAPHY RESULTS - article

The method of computer photodensitometry for evaluation of  thin-layer chromatography outcomes is offered.  An image of a plate surface is digitalized  with  scanner. The digital image is mathematically processed for determination of  the square of a spot, square on half-heights and integrated optical intensity. By this way it is automatically calculated an  amount of tested substance. Mathematically calculated parameters of spot fronts can be used for additional identification of substances and for analysis of mathematically calculated complicated chromatography images. There has been created a computer program “DensitoAnalyze” for this purpose.