Doroshchuk V. A., Gonta N. A., Kulichenko S. A. Determination of torasemide in biological fluids by HPLC pre-phenol-induced micellar extraction concentrating

2011 – Zurnal Hromatografichnogo Tovarystva  – V. ХІ. – № 1-4. –  P. 34-41

UDC 543.544.5.068.7. 542.61. 611.185.1

DOROSHCHUK V. A., GONTA N. A., KULICHENKO S. A.

DETERMINATION OF TORASEMIDE IN BIOLOGICAL FLUIDS BY HPLC PRE-PHENOL-INDUCED MICELLAR EXTRACTION CONCENTRATING - article

The procedure for determining of  torasemide in urine by the method of reversed-phase high-performance liquid chromatography (RP HPLC) with preconcentrating by micellar extraction has been developed. It has been shown that the quantitative recovery of the preparation attains using the hybrophobic micellar phases of non-ionic surfactant (NS) modified with phenol. Under the optimal conditions (preconcentration of 80 mL of sample in the presence of 8·10^-3 Mol L^-1 of Triton X-100 and 8·10^-2 Mol L^-1 of phenol)  the limit of detection of torasemide is 1.1 mMol L^-1.

Keywords: micellar extraction, preconcentrating, non-ionic surfactant, phenol, high-performance liquid chromatography, urine.