Levin M. G., Mirny А. V., Trutaev I. V., Ostanina N. V., Ishchenko N. V., Ginkul I. G., Osipov А. V., Markov V. V., Loginova L. P., Boichenko A. P. The application of singular values for the estimation of chromatographic peaks purity by using high-perfor

2010 – Zurnal Hromatografichnogo Tovarystva  – V. Х. – № 1-4. – P. 4-15

UDC 543.544.5.068.7 

LEVIN M. G., MIRNY А. V., TRUTAEV I. V., OSTANINA N. V., ISHCHENKO N. V., GINKUL I. G., OSIPOV А. V., MARKOV V. V., LOGINOVA L. P., BOICHENKO A. P.

THE APPLICATION OF SINGULAR VALUES FOR THE ESTIMATION OF CHROMATOGRAPHIC PEAKS PURITY BY USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE-ARRAY DETECTOR DATA - article

On the basis of the analysis of dependence of first ten singular values of spectrogram matrix of indopamide with the impurity of amlodipine, obtained in reversed-phase high-performance liquid chromatography mode (RP-HPLC) with diode-array detector, the correlation between the value of 5^th singular value and mass fraction of impurity in the range from 0.01 to 0.05% was observed. The results were proved by the analysis of simulated spectrograms of indopamide and amlodipine. The disclosed relationship could be useful for the determination of peaks purity in HPLC. 

Keywords: peak purity, HPLC, diode-array detector, singular value, purity factor, correlation.