Karbovskaya R. V., Boris I. I. The identification of anthocyanins by HPLC as the method of confirmation of authenticity of the fruit and berry stuff and ready produce

2008 – Zurnal Hromatografichnogo Tovarystva – V. VІІI. – № 3, 4. – P. 13-33

UDC 543.544.5.068.7. 543.61

KARBOVSKAYA R. V., BORIS I. I.

THE IDENTIFICATION OF ANTHOCYANINS BY HPLC AS THE METHOD OF CONFIRMATION OF AUTHENTICITY OF THE FRUIT AND BERRY STUFF AND READY PRODUCE - article

The method of qualitative determination of anthocyanins by HPLC has been developed. The method was implemented with the use of spectrophotometric detector. The identification was accomplished by the retention time of anthocyanins signals in visible area, and by the comparison of chromatogram profiles for different species of fruits and berries. The anthocyanins content of juice samples squashed from fresh fruits and berries and renewed from concentrates has been analyzed. The given method of the determination of anthocyanins content of colored by pigments fruit and berry stuff and ready produce gives reliable data about qualitative content of the product. The chromatograms of adulterated produce are given.