K. KRUPCZYNSKA, R. M. GADZALA-KOPCIUCH, B. BUSZEWSKI SELENIUM-CONTAINING PEPTIDES AS SPECIFIC ANALYTES FOR HPLC STATIONARY PHASES EVALUATION

2006 – Zurnal  Hromatografichnogo Tovarystva – V. VI – № 1. – P. 4-13

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K. KRUPCZYNSKA, R. M. GADZALA-KOPCIUCH, B. BUSZEWSKI

SELENIUM-CONTAINING PEPTIDES AS SPECIFIC ANALYTES FOR HPLC STATIONARY PHASES EVALUATION - article

A stationary phase surface overage homogeneity and density assure high quality of chromatographic separation. Chemical character and structure of chemically formed layer onthe silica gel surface determine interactions between stationary phase, analyte and mobile phase during chromatographic process. Retention mechanisms (partition, adsorption, ion exchange, steric features), selectivity and resolution are consequence of the stationary phases properties.

The aim of the present studies was to determine the heterogeneity of the packing surface on the basis of physicochemical investigations and chromatographic tests. A series of packings with octadecyl chains chemically bonded into a silica gel were prepared for this purpose. ²⁹Si CP/MAS NMR spectra and chromatographic measurements results were the base for simple topographical model of the chemically modified stationary surface. Water-soluble selenium-containing peptides (833 Da and 2607 Da) were used for the evaluation oflaboratory-prepared octadecyl stationary phases with high and low coverage density before and after end-capping. The aim of this work was to study the differences in the surface coverage density and homogeneity and conformation changes of chemically bonded moieties and the influence of these parameters on the separation of selenopeptides mixture.