Статті

SYROTCHUK O.A., DIDUKH I.R., ZAITSEV V.M. FEATURES OF TRIMEBUTIN AND PARABENS SEPARATION BY LIQUID CHROMATOGRAPHY USING STATIONARY PHASES OF DIFFERENT SELECTIVITY

2012 – Zurnal Hromatografichnogo Tovarystva – V. ХІІ. – № 1-4. – P. 4-12 

UDC 543.544.5.068.7:615.074 

SYROTCHUK O.A., DIDUKH I.R., ZAITSEV V.M. 

FEATURES OF TRIMEBUTIN AND PARABENS SEPARATION BY LIQUID CHROMATOGRAPHY USING STATIONARY PHASES OF DIFFERENT SELECTIVITY 

The article is about chromatografic behavior of the compounds of different chemical nature (trimebutine, methylparaben, propylparaben, gallic acid, 4-hydroxy benzoic acid) depending on pH of the mobile phases used on chromatographic columns of different selectivity (C18, phenyl, pentafluorophenyl). It has been shown that retention factor of trimebutine, increases dramatically if pH chahges from 2.1 to 6.8. Retention factor increases from 0.6 to 19.0 for C18-column. Retention factor increases less (from 0.2 to 6.8) when pentafluorophenyl column is used. Hence, pentafluorophenyl column is less sensitive to pH changes. So, it makes methods developed using this column more robust.

Keywords: trimebutine, parabens, HPLC, phenyl-column, pentafluorophenyl-column.