Articles

I.N. Golets, V.V. Levchyk, S.O. Alekseev, M.F. Zui Solid-phase extraction with C18 silica gel modified with 2,4-dinitrophenylhydrazine and HPLC-UV determination of benzaldehyde and furfural in beverages

UDK 543.31+54-414+661.183.7]:663.8
DOI:

Golets I.N., Levchyk V.V., Alekseev S.O., Zui M.F.

Solid-phase extraction with C18 silica gel modified with 2,4-dinitrophenylhydrazine and HPLC-UV determination of benzaldehyde and furfural in beverages

A method of solid-phase extraction (SPE) of aromatic aldehydes, in combination with HPLC/UV determination, has been developed for the analysis of soft drinks. A SPE cartridge was filled with C18 silica gel impregnated with 2,4-dinitrophenylhydrazine (DNPH). The developed method includes the steps of sorption, derivatization, and elution of carbonyl compounds, which were carried out sequentially in dynamic mode. The following parameters were optimized: type of sorbent, mineral acid and its concentration, concentration of DNPH on the sorbent, and volume of aqueous sample. When an aqueous solution of aldehydes was passed through the SPE cartridge, aromatic aldehydes were sorbed on the sorbent and converted into DNPH-hydrazones, then the derivatives were eluted with acetonitrile and determined by HPLC/UV. The limit of detection using the 3s-criterion was 1-2 mg/L for 2-furaldehyde and benzaldehyde. 2-furaldehyde was determined in ground and instant coffee. The method of standard additions was applied to eliminate the matrix effects. The relative standard deviation did not exceed 2.5%. The advantages of the sample preparation method of aromatic aldehydes are rapidity, simplicity, small amount of initial sample, economy of toxic organic solvents, and sufficiently high selectivity of determination.
Keywords: solid-phase extraction; sorption; silica gel impregnated with 2,4dinitrophenylhydrazine; aromatic aldehydes; analysis in soft drink; HPLC-UV.

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