Articles

Vavrinevych O.P., Hyrenko D.B., Hyrenko T.V., Omelchuk S.T., Syrota A.V. Improvement of analytical determination of residual quantities of active substances of the triasole class in juices

UDC 632.95.028:543.06: [635.1/.7+634.7+633.5/.8]
DOI: 10.15407/zht2020.66.024

Vavrinevych O.P., Hyrenko D.B., Hyrenko T.V., Omelchuk S.T., Syrota A.V.

Improvement of analytical determination of residual quantities of active substances of the triasole class in juices

Today, the most common among fungicides approved for use in Ukraine, as well as in other countries, are compounds of the triazole class. Juices, as a product of processing agricultural raw materials, can be consumed by a wide range of the population. The purpose of work was to develop analytical methods for compound of triazole (difenoconazole and propiconazole) in juices to ensure quality control of agricultural products grown with the use of these fungicides.
Conditions for chromatography of difenoconazole and propiconazole using gas-liquid chromatography with capillary column SH-Rxi-5ms (30 m 0,25 mm) and electron capture detector were determined. Optimal conditions of difenoconazole chromatography were established: thermostat column temperature - 280 C, evaporator temperature - 290 C, detector thermostat temperature - 300 C. Retention time: isomer 1 - (6,91 +- 0,1) minutes, isomer 2 - (7,04 +- 0,1) minutes. The linear range of detection is 0,01-0,04 ug/cm3. The optimal conditions for chromatography of propiconazole were also established: the temperature of the column thermostat in the programming mode: initial T - 100 C with an exposure of 1 minute; from 90 C to 260 C - rate of temperature rise 40 C/min; exposure - 7,45 minutes; evaporator temperature - 270 C, detector temperature - 280 C. The retention time under these conditions was 3,49 +- 0,1 minutes for isomer 1 and 3,57 +- 0,1 minutes for isomer 2. The linear range of detection is 0,01-0,05 ug/cm3.
The calibration dependence of the area of peaks of the investigated substances on their concentration was established and described by the linear regression equation.
Key words: difenoconazole, propiconazole, GAS-liquid chromatography, fungicides, triazole, juice.

REFERENCES

1. Perelik pestytsydiv i ahrokhimikativ, dozvolenykh do vykorystannya v Ukrayini (Ofitsiyne vydannya) Kyyiv: Yunivest Media, 2020, 895 C.
2. Sandra M. Garland, Robert C. Menary, Noel W. Davies Dissipation of Propiconazole and Tebuconazole in Peppermint Crops (Mentha piperita (Labiatae)) and Their Residues in Distilled Oils J. Agric. Food Chem. 1999, 47, 1, P. 294-298. DOI: 10.1021/jf980120e
3. Shen W.I., Yang W., Shen C., Zhao Z., Xu J., Ding T. Determination of difenoconazole residue in foods by gas chromatography-negative chemical ionization mass spectrometry. Chinese journal of chromatography. 2007. May. 25(3). P. 418-421.
4. T. Kadifkova Panovska, Z. Kavrakovski, S. Bauer Determination of propiconazole residues in tomatoes by gas chromatography Bulletin of the Chemists and Technologists of Macedonia, Vol 19, No 1, P. 27-33.
5. Novokhatska O.O., Lipavska A.O., Korshun O.M., Vavrinevych O.P., Omelchuk S.T. Analitychne zabezpechennya hihiyenichnoho kontrolyu zalyshkovykh kilkostey flufenatsetu v ob`yektakh navkolyshnoho seredovyshcha ta kartopli. Zhurnal Khromatohrafichnoho tovarystva. 2018. Tom XVII, S. 17-24. DOI: 10.15407/zht2018.64.017
6. PPDB: Pesticide Properties Data Base. URL: http://sitem.herts.ac.uk/aeru/footprint/en/
7. DSTU ISO 8466-1-2001. Yakist vody. Vyznachannya hraduyuvalnoyi kharakterystyky metodyk kilkisnoho khimichnoho analizu. Chastyna 1. Statystychne otsinyuvannya liniynoyi hraduyuvalnoyi kharakterystyky (ISO 8466-1:1990, IDT). 10 S.
8. Pro vykorystannya norm tochnosti i pravylnosti vymiryuvan pry zdiysnenni kontrolyu za vmistom khimichnykh rechovyn v prodovolchiy syrovyni, produktakh kharchuvannya i ob`yektakh dovkillya i vidpovidnosti mizh velychynamy MDR i HDK i hranytsyamy analitychnoho vyznachennya khimichnykh rechovyn. Postanova MOZ Ukrayiny. N 20, 20.04.1999.