Articles
Vavrinevych O.P., Hyrenko D.B., Hyrenko T.V., Omelchuk S.T., Syrota A.V. Improvement of analytical determination of difenoconazole residual quantities in vegetable, berries and technical cultures
UDC 632.95.028:543.06: [635.1/.7+634.7+633.5/.8]
DOI: 10.15407/zht2019.65.024
articleVavrinevych O.P., Hyrenko D.B., Hyrenko T.V., Omelchuk S.T., Syrota A.V.
IMPROVEMENT OF ANALYTICAL DETERMINATION OF DIFENOCONAZOLE RESIDUAL QUANTITIES IN VEGETABLE, BERRIES AND TECHNICAL CULTURES
Development of methods and improvement of analytical controls of pesticides residues in agricultural products – is an important step in pre-registration research of pesticides. The purpose of work was to develop analytical methods for difenoconazole, compound from triazole chemical class, determination in the vegetable (cabbage, carrots, onions), berry (strawberries, watermelon) and industrial crops (soybeans) for monitoring the safety of agricultural products grown using fungicides based on triazole compounds.
Conditions for chromatography of difenoconazole using gas-liquid chromatography with a glass packed column (1000 mm x 3 mm, 5% SE-30) and capillary columns SH-Rxi-5ms (30 m 0,32 mm), DB-5 (30 m 0,32 mm were determined. The best results are obtained when used capillary column SH-Rxi-5ms (30 m 0,32 mm). Optimal conditions of difenoconazole chromatography were established: thermostat column temperature - 280 C, evaporator temperature – 290 C, detector thermostat temperature– 300 C. Retention time – 1 isomer - (6.85 ± 0.1) minutes, 2 isomer - (6.98 ± 0.1) minutes. The calibration dependence of the area of peaks of the investigated substances on its concentration is established and is described by linear regression equations.
Key words: difenoconazole, GAS-liquid chromatography, fungicides, triazole, vegetable, berry and industrial crops.
REFERENCES
1. Perelik pestytsydiv i ahrokhimikativ, dozvolenykh do vykorystannya v Ukrayini (Ofitsiyne vydannya) Rafalskyy V.V., Vashchenko, V.M., Chaykovska, V.V., Koretskyy A.P. Kyiv: Yunivest Media, 2018, 1039 c.
2. Dopovnennya do Pereliku pestytsydiv i ahrokhimikativ, dozvolenykh do vykorystannya v Ukrayini (Ofitsiyne vydannya) Rafalskyy V.V., Vashchenko V.M, Chaykovska V.V. Kyiv: Yunivest Media, 2019, 591 c.
3. Shen W., Yang W, Shen C, Zhao Z, Xu J, Ding T. Determination of difenoconazole residue in foods by gas chromatography-negative chemical ionization mass spectrometry. Chinese journal of chromatography. 2007. May. 25(3). P. 418-421.
4. Novohatska O.O., Milohov D.S., Vavrinevych O.P., Omelchuk S.T., Antonenko A.M. Analytical support of hygienic control of oxathiapiprolin residual amounts in environmental objects and potatoes. Medical and Clinical Chemistry. 2017. Vol.19. No 3. P. 5-10. DOI 10.11603/mcch.2410-681X.2017.v0.i3.8189. https://doi.org/10.11603/mcch.2410-681X.2017.v0.i3.8189
5. Novokhatska O.O., Lipavska A.O., Korshun O.M., Vavrinevych O.P., Omelchuk S.T. Analitychne zabezpechennya hihiyenichnoho kontrolyu zalyshkovykh kilkostey flufenatsetu v obyektakh navkolyshnoho seredovyshcha ta kartopli. Zurnal Hromatograficnogo tovaristva. 2018. Vol. XVII, S. 17-24. DOI: 10.15407/zht2018.64.017 https://doi.org/10.15407/zht2018.64.017
6. PPDB: Pesticide Properties Data Base. URL: http://sitem.herts.ac.uk/aeru/footprint/en/
7. DSTU ISO 8466-1-2001. Yakist vody. Vyznachannya hraduyuvalnoyi kharakterystyky metodyk kilkisnoho khimichnoho analizu. Chastyna 1. Statystychne otsinyuvannya liniynoyi hraduyuvalnoyi kharakterystyky (ISO 8466-1:1990, IDT). 10 s.
8. Pro vykorystannya norm tochnosti i pravylnosti vymiryuvan pry zdiysnenni kontrolyu za vmistom khimichnykh rechovyn v prodovolchiy syrovyni, produktakh kharchuvannya i obʺyektakh dovkillya i vidpovidnosti mizh velychynamy MDR i HDK ta hranytsyamy analitychnoho vyznachennya khimichnykh rechovyn: Postanova MOZ Ukrayiny vid 20.04.1999 r. No 20.