Articles

Vavrinevych O.P., Hyrenko D.B., Hyrenko T.V., Omelchuk S.T., Syrota A.V. Improvement of analytical determination of difenoconazole residual quantities in vegetable, berries and technical cultures

UDC 632.95.028:543.06: [635.1/.7+634.7+633.5/.8]
DOI: 10.15407/zht2019.65.024

Vavrinevych O.P., Hyrenko D.B., Hyrenko T.V., Omelchuk S.T., Syrota A.V.

IMPROVEMENT OF ANALYTICAL DETERMINATION OF DIFENOCONAZOLE RESIDUAL QUANTITIES IN VEGETABLE, BERRIES AND TECHNICAL CULTURES

Development of methods and improvement of analytical controls of pesticides residues in agricultural products – is an important step in pre-registration research of pesticides. The purpose of work was to develop analytical methods for difenoconazole, compound from triazole chemical class, determination in the vegetable (cabbage, carrots, onions), berry (strawberries, watermelon) and industrial crops (soybeans) for monitoring the safety of agricultural products grown using fungicides based on triazole compounds.
Conditions for chromatography of difenoconazole using gas-liquid chromatography with a glass packed column (1000 mm x 3 mm, 5% SE-30) and capillary columns SH-Rxi-5ms (30 m  0,32 mm), DB-5 (30 m  0,32 mm were determined. The best results are obtained when used capillary column SH-Rxi-5ms (30 m  0,32 mm). Optimal conditions of difenoconazole chromatography were established: thermostat column temperature - 280 C, evaporator temperature – 290 C, detector thermostat temperature– 300 C. Retention time – 1 isomer - (6.85 ± 0.1) minutes, 2 isomer - (6.98 ± 0.1) minutes. The calibration dependence of the area of peaks of the investigated substances on its concentration is established and is described by linear regression equations.
Key words: difenoconazole, GAS-liquid chromatography, fungicides, triazole, vegetable, berry and industrial crops.

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