Articles

SYROTCHUK O.A., DIDUKH I.R., MARKIN R.O. ZAITSEV V.N. PARACETAMOL, PHENYLPROPANOLAMINE HYDROCHLORIDE, CAFFEINE, CHLORPHENIRAMINE MALEAT DETERMINATION IN PREPARATION AGAINST COMMON COLD USING EMBEDDED POLAR COLUMN

2014 – Zurnal Hromatografichnogo Tovarystva – V. ХІV. – № 1-4. – P. 5-18 

UDC 543.544.5.068.7:615.074

SYROTCHUK O.A., DIDUKH I.R., MARKIN R.O., ZAITSEV V.N. 

PARACETAMOL, PHENYLPROPANOLAMINE HYDROCHLORIDE, CAFFEINE, CHLORPHENIRAMINE MALEAT DETERMINATION IN PREPARATION AGAINST COMMON COLD USING EMBEDDED POLAR COLUMN  - article

A novel method of paracetamol, caffeine, phenylpropanolamine hydrochloride and chlorpheniramine mateate determination in common cold medical preparation has been developed and validated. Using embedded polar column Supelcosil LC-ABZ allowed us to avoid ion-pair agent in the mobile phase. The method in non-gradient and because of this it need twice less time than gradient methods. Embedded polar column and sampling allowed us 10-20 times to decrease organic solvent usage if compared to US pharmacopoeia methods. The method was validated with respect to linearity, precision, accuracy, selectivity, and robustness. The method was found to be applicable for routine analyzes (assays and stability tests) of paracetamol, caffeine, phenylpropanolamine hydrochloride and chlorpheniramine mateate.

Keywords: paracetamol, phenylpropanolamine, caffeine, chlorpheniramine, validation.