Articles

MELNYCHUK S.D., DV GRYNCHUK D.V., TSVILIHOVSKYY V.I. COMPARATIVE ANALYSIS OF INDIRECT METHODS OF HPLC-QUANTITATION OF AMINO ACIDS AND GENERAL METHODICAL TRENDS AND PROSPECTS OF AMINO ACID ANALYSIS

2013 – Zurnal Hromatografichnogo Tovarystva – V. ХІІІ. – № 1-4. – P. 4-24.

UDC 577.112.37:543.42/.544

MELNYCHUK S.D., GRYNCHUK D.V., TSVILIHOVSKYY V.I. 

COMPARATIVE ANALYSIS OF INDIRECT METHODS OF HPLC-QUANTITATION OF AMINO ACIDS AND GENERAL METHODICAL TRENDS AND PROSPECTS OF AMINO ACID ANALYSIS - article

The review analyzed the achievements and  possibilities of application of indirect (derivatizing) methods of quantitative amino acid analysis of feed, food and other objects using different derivatizing reagents, and various  kinds of liquid chromatography, and detectors. Examples of practical application of derivatizing methods in analytical studies of samples with complex matrices and requirements for these regulations are cited. Systematized review of characteristics of methods and its applications was made. It was shown the current trends and made a forecast for the possible prospects of methodological development of chiral and achiral chromatographic and spectral methods for amino acid analysis.

Keywords: amino acids methods amino acid analysis, methods of derivatization of amino acids, deryvatyzatsiyni reagents, high  performance liquid chromatography, chiral chromatography, feed, food.